{"id":26400,"date":"2022-05-09T16:56:50","date_gmt":"2022-05-09T08:56:50","guid":{"rendered":"https:\/\/www.accscicn.com\/?page_id=26400"},"modified":"2022-10-26T15:42:14","modified_gmt":"2022-10-26T07:42:14","slug":"ultrafast_fluorescence_upconversion_spectroscopy","status":"publish","type":"page","link":"https:\/\/www.accscicn.com\/en\/product\/ultrafast_fluorescence_upconversion_spectroscopy\/","title":{"rendered":"Ultrafast fluorescence upconversion spectroscopy system"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"26400\" class=\"elementor elementor-26400\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-49b125d6 elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"49b125d6\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div 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768w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/um100-300x200.png 300w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/um100-1024x683.png 1024w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/um100-1536x1024.png 1536w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/um100-2048x1366.png 2048w\" sizes=\"(max-width: 768px) 100vw, 768px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-6b1cd598\" data-id=\"6b1cd598\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7d872ba elementor-widget elementor-widget-uael-advanced-heading\" data-id=\"7d872ba\" data-element_type=\"widget\" data-widget_type=\"uael-advanced-heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\r\n\t\t<div class=\"uael-module-content uael-heading-wrapper\">\r\n\t\t\t\t\t\t\t\t\t\t<div class=\"uael-sub-heading elementor-inline-editing\" data-elementor-setting-key=\"sub_heading\" data-elementor-inline-editing-toolbar=\"advanced\" ><p>Structure, function and reaction kinetics of macromolecules in chemical biology*<\/p><\/div>\r\n\t\t\t\r\n\t\t\t<h1 class=\"uael-heading\">\r\n\t\t\t\t\t\t\t\t\t\t<span class=\"uael-heading-text elementor-inline-editing uael-size--default\" data-elementor-setting-key=\"heading_title\" data-elementor-inline-editing-toolbar=\"basic\"><b>Ultrafast fluorescence upconversion spectroscopy system\n<\/b><br>Fluorescence Upconversion Spectroscopy<\/span>\r\n\t\t\t\t\t\t\t<\/h1>\r\n\r\n\t\t\t\r\n\t\t\t\t\t\t<div class=\"uael-module-content uael-separator-parent\">\r\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"uael-separator\"><\/div>\r\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\r\n\t\t\t\t\t\t\t<div class=\"uael-subheading elementor-inline-editing\" data-elementor-setting-key=\"heading_description\" data-elementor-inline-editing-toolbar=\"advanced\" >\r\n\t\t\t\t\t<p>In terms of temporal resolution, measurement sensitivity and accuracy, fluorescence upconversion is ultimately the most competitive technology.<br><span style=\"font-style: inherit;font-weight: inherit;background-color: var( --e-global-color-astglobalcolor5 )\">The technique has been used to study the ultraviolet, visible and near-infrared spectral regions and has been used to study many different phenomena such as solvation kinetics, intramolecular coherence vibrations, ultrafast photoisomerization reaction kinetics, charge transfer reactions, fluorescence properties of DNA nucleosides and nucleotides, solvation reactions at multiple sites in globular proteins, as well as water kinetics in biological recognition, and condensed matter.<\/span><\/p><p><span style=\"background-color: var( --e-global-color-astglobalcolor5 )\"><\/span><\/p>\t\t\t\t<\/div>\r\n\t\t\t\t\r\n\t\t\t\t\t\t<\/div>\r\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7263d707 elementor-widget elementor-widget-button\" data-id=\"7263d707\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/www.accscicn.com\/en\/contact-us\/\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Inquire for a quote<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-45a85d68 elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"45a85d68\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-28200955\" data-id=\"28200955\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-54185cfb elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"54185cfb\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-1c678f67\" data-id=\"1c678f67\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-35c93ea1 elementor-tabs-alignment-stretch elementor-tabs-view-horizontal elementor-widget elementor-widget-tabs\" data-id=\"35c93ea1\" data-element_type=\"widget\" data-widget_type=\"tabs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.21.0 - 20-05-2024 *\/\n.elementor-widget-tabs.elementor-tabs-view-vertical .elementor-tabs-wrapper{width:25%;flex-shrink:0}.elementor-widget-tabs.elementor-tabs-view-vertical .elementor-tab-desktop-title.elementor-active{border-inline-end-style:none}.elementor-widget-tabs.elementor-tabs-view-vertical .elementor-tab-desktop-title.elementor-active:after,.elementor-widget-tabs.elementor-tabs-view-vertical 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.elementor-tab-desktop-title.elementor-active:before{right:100%}.elementor-widget-tabs.elementor-tabs-view-horizontal .elementor-tab-desktop-title.elementor-active:after{left:100%}.elementor-widget-tabs .elementor-tab-content,.elementor-widget-tabs .elementor-tab-title,.elementor-widget-tabs .elementor-tab-title:after,.elementor-widget-tabs .elementor-tab-title:before,.elementor-widget-tabs .elementor-tabs-content-wrapper{border:1px #d5d8dc}.elementor-widget-tabs .elementor-tabs{text-align:start}.elementor-widget-tabs .elementor-tabs-wrapper{overflow:hidden}.elementor-widget-tabs .elementor-tab-title{cursor:pointer;outline:var(--focus-outline,none)}.elementor-widget-tabs .elementor-tab-desktop-title{position:relative;padding:20px 25px;font-weight:700;line-height:1;border:solid transparent}.elementor-widget-tabs .elementor-tab-desktop-title.elementor-active{border-color:#d5d8dc}.elementor-widget-tabs .elementor-tab-desktop-title.elementor-active:after,.elementor-widget-tabs .elementor-tab-desktop-title.elementor-active:before{display:block;content:\"\";position:absolute}.elementor-widget-tabs .elementor-tab-desktop-title:focus-visible{border:1px solid #000}.elementor-widget-tabs .elementor-tab-mobile-title{padding:10px;cursor:pointer}.elementor-widget-tabs .elementor-tab-content{padding:20px;display:none}@media (max-width:767px){.elementor-tabs .elementor-tab-content,.elementor-tabs .elementor-tab-title{border-style:solid solid none}.elementor-tabs .elementor-tabs-wrapper{display:none}.elementor-tabs .elementor-tabs-content-wrapper{border-bottom-style:solid}.elementor-tabs .elementor-tab-content{padding:10px}}@media (min-width:768px){.elementor-widget-tabs.elementor-tabs-view-vertical .elementor-tabs{display:flex}.elementor-widget-tabs.elementor-tabs-view-vertical .elementor-tabs-wrapper{flex-direction:column}.elementor-widget-tabs.elementor-tabs-view-vertical .elementor-tabs-content-wrapper{flex-grow:1;border-style:solid solid solid none}.elementor-widget-tabs.elementor-tabs-view-horizontal .elementor-tab-content{border-style:none solid solid}.elementor-widget-tabs.elementor-tabs-alignment-center .elementor-tabs-wrapper,.elementor-widget-tabs.elementor-tabs-alignment-end .elementor-tabs-wrapper,.elementor-widget-tabs.elementor-tabs-alignment-stretch .elementor-tabs-wrapper{display:flex}.elementor-widget-tabs.elementor-tabs-alignment-center .elementor-tabs-wrapper{justify-content:center}.elementor-widget-tabs.elementor-tabs-alignment-end .elementor-tabs-wrapper{justify-content:flex-end}.elementor-widget-tabs.elementor-tabs-alignment-stretch.elementor-tabs-view-horizontal .elementor-tab-title{width:100%}.elementor-widget-tabs.elementor-tabs-alignment-stretch.elementor-tabs-view-vertical .elementor-tab-title{height:100%}.elementor-tabs .elementor-tab-mobile-title{display:none}}<\/style>\t\t<div class=\"elementor-tabs\">\n\t\t\t<div class=\"elementor-tabs-wrapper\" role=\"tablist\" >\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-9021\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"elementor-tab-content-9021\" aria-expanded=\"false\">Overview<\/div>\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-9022\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-9022\" aria-expanded=\"false\">Parameters<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t\t<div class=\"elementor-tabs-content-wrapper\" role=\"tablist\" aria-orientation=\"vertical\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"elementor-tab-content-9021\" aria-expanded=\"false\">Overview<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-9021\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"1\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-9021\" tabindex=\"0\" hidden=\"false\"><p>The ultrafast fluorescence upconversion spectroscopy system is a detection system for transient fluorescence spectroscopy and dynamics with femtosecond time-resolved scales constructed in combination with a femtosecond laser light source. The operating principle is shown in the figure on the right. First, the femtosecond laser light source is split into two beams, one beam is used to excite the sample, and the generated fluorescence is collected and converged into a linear crystal with the second femtosecond laser (gated pulsed laser) to generate a sum frequency signal. The delay time between the two laser beams is controlled by the optical delay line, and the sum frequency signal at different delay times reflects the fluorescence intensity at that moment, thus realizing the acquisition of fluorescence decay signal at the femtosecond scale.<img decoding=\"async\" class=\"alignnone  wp-image-26405\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/Ultrafast-Fluorescence-Upconversion-Spectroscopy.png\" alt=\"\" width=\"520\" height=\"291\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/Ultrafast-Fluorescence-Upconversion-Spectroscopy.png 860w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/Ultrafast-Fluorescence-Upconversion-Spectroscopy-300x168.png 300w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/Ultrafast-Fluorescence-Upconversion-Spectroscopy-768x430.png 768w\" sizes=\"(max-width: 520px) 100vw, 520px\" \/><img decoding=\"async\" class=\"alignnone  wp-image-26406\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/\u5fae\u4fe1\u622a\u56fe_20220506104733.png\" alt=\"\" width=\"581\" height=\"550\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/\u5fae\u4fe1\u622a\u56fe_20220506104733.png 661w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/\u5fae\u4fe1\u622a\u56fe_20220506104733-300x284.png 300w\" sizes=\"(max-width: 581px) 100vw, 581px\" \/><\/p><\/div>\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-9022\" aria-expanded=\"false\">Parameters<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-9022\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"2\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-9022\" tabindex=\"0\" hidden=\"hidden\"><ol><li style=\"list-style-type: none;\"><ol><li>Fluorescence wavelength detection range: 360nm-900nm<\/li><li>Single grating monochromator with high sensitivity PMT detector + gated photon counter<\/li><li>High-quality frequency combining crystals equipped with electric angle rotary table; automatic control of frequency combining angle<\/li><li>Detection time window: 8 ns<\/li><li>IRF (instrument response function): \u22641ps<\/li><li>Realize single wavelength kinetic detection and spectral scanning + kinetic 3D detection mode<\/li><li>High-speed optical delay line: the fastest speed of optical delay line 400mm\/s, accuracy 0.1<\/li><\/ol><\/li><\/ol><\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-24beb21a elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"24beb21a\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-5cac8865\" data-id=\"5cac8865\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-26a3f702 elementor-widget elementor-widget-text-editor\" data-id=\"26a3f702\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.21.0 - 20-05-2024 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#69727d;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#69727d;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p><strong>Related Applications<\/strong><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-2557c8bf elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2557c8bf\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-933e96c\" data-id=\"933e96c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>\u5316\u5b66\u751f\u7269\u5b66\u5927\u5206\u5b50\u7ed3\u6784\u3001\u529f\u80fd\u548c\u53cd\u5e94\u52a8\u529b\u5b66\u7814 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