{"id":26578,"date":"2022-05-10T10:53:56","date_gmt":"2022-05-10T02:53:56","guid":{"rendered":"https:\/\/www.accscicn.com\/?p=26578"},"modified":"2022-10-20T17:33:20","modified_gmt":"2022-10-20T09:33:20","slug":"application-examples-of-fluorescence-imaging-systems","status":"publish","type":"post","link":"https:\/\/www.accscicn.com\/en\/insight\/application-examples-of-fluorescence-imaging-systems\/","title":{"rendered":"Application examples of fluorescence imaging systems"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"26578\" class=\"elementor elementor-26578\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-bbb8c22 elementor-section-full_width elementor-section-stretched elementor-section-height-default elementor-section-height-default\" data-id=\"bbb8c22\" data-element_type=\"section\" data-settings=\"{&quot;stretch_section&quot;:&quot;section-stretched&quot;,&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-5bd27f2e\" data-id=\"5bd27f2e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-5b48d553 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5b48d553\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-76e8a861\" data-id=\"76e8a861\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4786d7f7 elementor-widget elementor-widget-heading\" data-id=\"4786d7f7\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.21.0 - 20-05-2024 *\/\n.elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}<\/style><h3 class=\"elementor-heading-title elementor-size-default\">Application examples of fluorescence imaging systems explained<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-64b5c578 elementor-widget elementor-widget-text-editor\" data-id=\"64b5c578\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.21.0 - 20-05-2024 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#69727d;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#69727d;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p>The multi-functional scanner fluorescence imaging system is a high-precision instrument that uses laser precision scanning, time-resolved acquisition and image processing techniques to obtain the photophysical properties of materials in the micro-nano scale space. It is mainly used to study the fluorescence kinetic process and imaging in the micro and nano scale of semiconductor samples, photoelectric conversion materials, photocatalytic materials and biological samples. Its main structure includes laser light source, microscope (ortho or inverted), laser scanning device and detector, etc. It can be fully automated, digital image acquisition and processing through computer control, and can realize various imaging and detection functions such as fluorescence intensity confocal imaging, fluorescence lifetime imaging, carrier migration imaging, micro-nano space fluorescence spectroscopy and Raman spectroscopy acquisition. The system can also be combined with low-temperature device, high-voltage device, (transient) photocurrent\/photovoltage detection device and pulse voltage device to achieve a variety of external field conditions of fluorescence kinetics detection, high spatial resolution of photocurrent imaging, electroluminescence kinetics and imaging and other special functions.<\/p><p>01<strong>Working schematic<\/strong><\/p><p><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone size-full wp-image-26583\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-1-1.png\" alt=\"\" width=\"1080\" height=\"560\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-1-1.png 1080w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-1-1-300x156.png 300w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-1-1-1024x531.png 1024w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-1-1-768x398.png 768w\" sizes=\"(max-width: 1080px) 100vw, 1080px\" \/><\/p><p>02<strong>Software acquisition interface<\/strong><\/p><p><img decoding=\"async\" class=\"alignnone size-full wp-image-26581\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-16.png\" alt=\"\" width=\"1080\" height=\"662\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-16.png 1080w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-16-300x184.png 300w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-16-1024x628.png 1024w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-16-768x471.png 768w\" sizes=\"(max-width: 1080px) 100vw, 1080px\" \/><\/p><p><strong>Achievable functions<\/strong><\/p><ul><li>Fluorescence intensity (lifetime) imaging<\/li><li>Selectable scanning of pixel points<\/li><li>Settable imaging scan speed<\/li><li>Spot position programmable<\/li><li>Adjustable scanning area and magnification<\/li><li>Fluorescence lifetime auto-fitting<\/li><li>Fixed-point excitation to observe carrier diffusion<\/li><li>All data can be exported<\/li><\/ul><p>03<strong>Fluorescence imaging scan video demonstration<\/strong><\/p><p><img decoding=\"async\" class=\"alignnone wp-image-26582\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-1.gif\" alt=\"\" width=\"250\" height=\"247\" \/> \u00a0 <img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26584\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-2.gif\" alt=\"\" width=\"270\" height=\"243\" \/><\/p><p>Laser fast scanning video under the microscope Fluorescence imaging scanning video of chalcogenide thin film<\/p><p>(2x accelerated playback) (8x accelerated playback)<\/p><p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26586\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-3.gif\" alt=\"\" width=\"270\" height=\"270\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26588\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-4.gif\" alt=\"\" width=\"270\" height=\"270\" \/><\/p><p>Calcium titanite nanowire fluorescence imaging scan video Plant cell fluorescence imaging scan video<\/p><p>(2x accelerated playback) (2x accelerated playback)<\/p><p>04<strong>Main technical parameters of the system<\/strong><\/p><p><strong>I. Laser scanning oscillator module .<\/strong><\/p><p>1, laser fiber input, distribution control diaphragm system (regulation range: 25\u03bcm-2mm)<\/p><p>2, laser scanning imaging range: 256 \u00d7 256 pixel dots, up to 4096 \u00d7 4096 pixel dots<\/p><p>3\u3001Imaging magnification (zoom): 1-32 times<\/p><p>4\u3001Minimum pixel dwell time: 2.1\u03bcs<\/p><p><strong>II. TCSPC Module .<\/strong><\/p><p>1\u3001Time accuracy: 7 ps<\/p><p>3\u3001Time window: 50 ns - 5 \u03bcs<\/p><p>4\u3001Instrument response function (IRF): \u2264 200 ps<\/p><p>5\u3001Spectrum detection range: 400-1000nm<\/p><p><strong>III. Steady-state spectral detection module .<\/strong><\/p><p>1\u3001Spectrometer (configuration can be adjusted according to customer needs)<\/p><p>2\u3001Focal length: 200mm<\/p><p>3, grating according to the user sample emission wavelength range optional<\/p><p>4\u3001Exit-coupled PMT detector or CCD<\/p><p>2\u3001Spectrum detection mode: wavelength scanning or CCD acquisition<\/p><p><strong>IV. Inverted microscope module .<\/strong><\/p><p>1, including lighting sources, dichroic, filters and other basic configuration<\/p><p>2, Objective: 100X, 50x, 10x, 5x (according to customer demand optional)<\/p><p>3\u3001Maximum spatial resolution: ~260nm (depending on objective and laser\/fluorescence wavelength)<\/p><p><strong>V. Lasers<\/strong>(Optional according to customer needs)<\/p><p><strong>VI. Photocurrent imaging module<\/strong>(Optional according to customer needs)<\/p><p><strong>VII. Electroluminescent module<\/strong>(Optional according to customer needs)<\/p><p><strong>Eight, low temperature \/ high pressure warehouse module<\/strong>(Optional according to customer needs)<\/p><p>05<strong>Application example 1: Fluorescence intensity (lifetime imaging)<\/strong><\/p><p>Fluorescence intensity and fluorescence lifetime imaging is the basic function of this system, which can perform fast confocal fluorescence imaging of micro-nano-scale samples or sample micro-nano-spatial structures with laser scanning, and can obtain fluorescence intensity and fluorescence lifetime spatial distribution information simultaneously.<\/p><p>Sample: MAPbI3 single crystal nanosheets and MAPbI3 nanowires<\/p><p>Experimental conditions: 100X air microscope, excitation wavelength: 405 nm<\/p><p>Imaging mode: Confocal laser scanning imaging mode<\/p><p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-26589\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-4-1.png\" alt=\"\" width=\"1080\" height=\"650\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-4-1.png 1080w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-4-1-300x181.png 300w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-4-1-1024x616.png 1024w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-4-1-768x462.png 768w\" sizes=\"(max-width: 1080px) 100vw, 1080px\" \/>\u00a0<\/p><p>Sample: Calcium titanite polycrystalline film of methylamine lead iodide<\/p><p>Experimental conditions: 100X (oil mirror), excitation wavelength: 405nm<\/p><p>Imaging mode: Confocal laser scanning imaging mode<\/p><p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26591\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-5-1.png\" alt=\"\" width=\"259\" height=\"235\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-5-1.png 675w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-5-1-300x272.png 300w\" sizes=\"(max-width: 259px) 100vw, 259px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26593\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-6-1.png\" alt=\"\" width=\"272\" height=\"256\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-6-1.png 671w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-6-1-300x283.png 300w\" sizes=\"(max-width: 272px) 100vw, 272px\" \/> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26595\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-7-1.png\" alt=\"\" width=\"311\" height=\"248\" \/> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26597\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-8-1.png\" alt=\"\" width=\"272\" height=\"273\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-8-1.png 595w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-8-1-300x300.png 300w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-8-1-150x150.png 150w\" sizes=\"(max-width: 272px) 100vw, 272px\" \/><\/p><p>By analyzing the fluorescence intensity change from a to b, the highest spatial resolution up to ~260 nm (the highest spatial resolution reaches the diffraction limit) can be obtained. Effective identification of complex structures such as grain boundaries in chalcogenide polycrystalline films<\/p><p>Samples: Plant cell specimens<\/p><p>Experimental conditions: 50X, excitation wavelength: 405nm<\/p><p>Imaging mode: Confocal laser scanning imaging mode<\/p><p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26598\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-9-1.png\" alt=\"\" width=\"257\" height=\"297\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-9-1.png 559w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-9-1-260x300.png 260w\" sizes=\"(max-width: 257px) 100vw, 257px\" \/> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26599\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-10-1.png\" alt=\"\" width=\"263\" height=\"302\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-10-1.png 565w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-10-1-261x300.png 261w\" sizes=\"(max-width: 263px) 100vw, 263px\" \/><\/p><p>Sample: 2D SnSe2 (weakly fluorescent material)<\/p><p>Experimental conditions: 100X (oil mirror), excitation wavelength: 405nm<\/p><p>Imaging mode: Confocal laser scanning imaging mode<\/p><p>\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26600\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-11-1.png\" alt=\"\" width=\"277\" height=\"234\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-11-1.png 607w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-11-1-300x253.png 300w\" sizes=\"(max-width: 277px) 100vw, 277px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26601\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-12-1.png\" alt=\"\" width=\"242\" height=\"229\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-12-1.png 527w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/05\/640-12-1-300x284.png 300w\" sizes=\"(max-width: 242px) 100vw, 242px\" \/><\/p><p><strong>Enables imaging of fluorescence intensity and lifetime of weakly fluorescent samples<\/strong><\/p><p><strong>Reference:<\/strong><\/p><p>Xing Zhou ,et al.,Tianyou Zhai**,Adv. Mater*. 2015, 27, 8035-8041<\/p><p>\u00a0<\/p><p>Source: Tristar Spectrum @ WeChat<\/p><p>Time: 2022\/05\/10<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-27b3c71\" data-id=\"27b3c71\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1cca7abf elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"1cca7abf\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.21.0 - 20-05-2024 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