{"id":26048,"date":"2022-02-21T18:18:07","date_gmt":"2022-02-21T10:18:07","guid":{"rendered":"https:\/\/www.accscicn.com\/?p=26048"},"modified":"2022-10-20T17:51:08","modified_gmt":"2022-10-20T09:51:08","slug":"app-mantis-2022022103","status":"publish","type":"post","link":"https:\/\/www.accscicn.com\/ko\/%ec%9d%b8%ec%82%ac%ec%9d%b4%ed%8a%b8\/%ec%95%a0%ed%94%8c%eb%a6%ac%ec%bc%80%ec%9d%b4%ec%85%98\/%ec%95%b1-%eb%a7%a8%ed%8b%b0%ec%8a%a4-2022022103\/","title":{"rendered":"MANTIS\u00ae \uc561\uccb4 \ud504\ub85c\uc138\uc11c\uc758 QuantiFluor\u00ae dsDNA \uc2dc\uc2a4\ud15c\uc744 \uc0ac\uc6a9\ud55c \ube60\ub974\uace0 \uc815\ud655\ud558\uba70 \uc720\uc5f0\ud55c DNA \uc815\ub7c9\ud654"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"26048\" class=\"elementor elementor-26048\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6cc1b2cb elementor-section-full_width elementor-section-stretched elementor-section-height-default elementor-section-height-default\" data-id=\"6cc1b2cb\" data-element_type=\"section\" data-settings=\"{&quot;stretch_section&quot;:&quot;section-stretched&quot;,&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-17adffd4\" data-id=\"17adffd4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-5a039472 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5a039472\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-609d0d23\" data-id=\"609d0d23\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-72410ec1 elementor-widget elementor-widget-heading\" data-id=\"72410ec1\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.21.0 - 20-05-2024 *\/\n.elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}<\/style><h3 class=\"elementor-heading-title elementor-size-default\">MANTIS\u00ae \uc561\uccb4 \ud504\ub85c\uc138\uc11c\uc758 QuantiFluor\u00ae dsDNA \uc2dc\uc2a4\ud15c\uc744 \uc0ac\uc6a9\ud55c \ube60\ub974\uace0 \uc815\ud655\ud558\uba70 \uc720\uc5f0\ud55c DNA \uc815\ub7c9\ud654\n<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-58cf909 elementor-widget elementor-widget-text-editor\" data-id=\"58cf909\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.21.0 - 20-05-2024 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#69727d;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#69727d;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p><strong>\ud504\ub808\uc820\ud14c\uc774\uc158<\/strong><\/p><p>\ud575\uc0b0 \uc815\ub7c9\ud654\ub294 \ubd84\uc790\uc0dd\ubb3c\ud559 \uc6cc\ud06c\ud50c\ub85c\uc6b0\uc5d0\uc11c \uc911\uc694\ud55c \ub2e8\uacc4\ub85c, \ud2b9\ud788 qPCR \ubc0f \ucc28\uc138\ub300 \uc2dc\ud000\uc2f1(NGS)\uacfc \uac19\uc740 \uace0\uae09 \uc560\ud50c\ub9ac\ucf00\uc774\uc158\uc758 \uacbd\uc6b0 \ub354\uc6b1 \uadf8\ub807\uc2b5\ub2c8\ub2e4. NGS\uc758 \uad81\uadf9\uc801\uc778 \ubaa9\ud45c\ub294 \ub9e4\uc6b0 \ubcf5\uc7a1\ud55c \ub77c\uc774\ube0c\ub7ec\ub9ac\ub97c \uc0dd\uc131\ud558\uace0 \ucd5c\ub300\ud55c\uc758 \uae4a\uc774\uc640 \uade0\uc9c8\uc131\uc73c\ub85c \uc2dc\ud000\uc2f1\ud558\ub294 \uac83\uc774\ubbc0\ub85c \uc815\ud655\ud55c \uc815\ub7c9\ud654\uac00 \ud2b9\ud788 \uc911\uc694\ud569\ub2c8\ub2e4. \ud575\uc0b0 \ub18d\ub3c4\ub97c \uc815\ud655\ud558\uac8c \uacb0\uc815\ud558\uae30 \uc704\ud55c \ub450 \uac00\uc9c0 \ud575\uc2ec \ub2e8\uacc4\ub294 \ub2e4\uc74c\uacfc \uac19\uc2b5\ub2c8\ub2e4:<\/p><ol><li>\ucd5c\ub300 \ud6a8\uc18c \ud6a8\uc728\uc740 \ub77c\uc774\ube0c\ub7ec\ub9ac \uc900\ube44 \uc804\uacfc 2) \uc2dc\ud000\uc2f1 \uc804 \ud480\ub9c1\uc744 \uc704\ud574 \uc0d8\ud50c\uc744 \ud45c\uc900\ud654\ud558\uae30 \uc704\ud55c \ub77c\uc774\ube0c\ub7ec\ub9ac \uc900\ube44 \ud6c4\uc5d0 \uc785\ub825\ub418\ub294 DNA\uc758 \uc591\uc5d0 \ubbfc\uac10\ud569\ub2c8\ub2e4. \ub610\ud55c \uc810\uc810 \ub354 \uc801\uc740 \uc591\uc758 \uc0d8\ud50c\uc744 \uc785\ub825\ud574\uc57c \ud558\uace0(\uc608: RNA-seq \uc2e4\ud5d8), \ub9ce\uc740 \uc77c\ubc18\uc801\uc778 \uc0d8\ud50c \uc720\ud615(\uc608: FFPE \ubc0f ccfDNA)\uc5d0\uc11c \ud575\uc0b0\uc744 \ubd84\ub9ac\ud574\uc57c \ud558\ub294 \uc5b4\ub824\uc6c0\uc73c\ub85c \uc778\ud574 \ub18d\ub3c4 \ucd94\uc815\uc774 \uc5b4\ub835\uc2b5\ub2c8\ub2e4.<\/li><\/ol><p>\uc5ec\ub7ec \uc815\ub7c9\ud654 \ubc29\ubc95\uc774 \uc874\uc7ac\ud558\uc9c0\ub9cc \ud615\uad11 \uc5fc\ub8cc \uae30\ubc18 \uc2dc\uc2a4\ud15c\uc740 \uac10\ub3c4, \ud45c\uc801 \uc120\ud0dd\uc131, \ube44\uc6a9 \ubc0f \uc0ac\uc6a9 \ud3b8\uc758\uc131\uc73c\ub85c \uc778\ud574 \uace0\uae09 \ub2e4\uc6b4\uc2a4\ud2b8\ub9bc \uc560\ud50c\ub9ac\ucf00\uc774\uc158\uc5d0 \uad8c\uc7a5\ub429\ub2c8\ub2e4. QuantiFluor\u00ae dsDNA \uc2dc\uc2a4\ud15c\uc744 \uc0ac\uc6a9\ud558\uba74 \ud615\uad11 \uc5fc\ub8cc \uc6d0\uc561\uc744 \ud76c\uc11d\ud558\uc5ec \uc791\uc5c5 \uc6a9\uc561\uc744 \ub9cc\ub4e4\uace0, \uc774\ub97c \uc18c\ub7c9\uc758 \uc815\uc81c\ub41c DNA \uc0d8\ud50c\uc774 \ub4e4\uc5b4 \uc788\ub294 \ub9c8\uc774\ud06c\ub85c\uc2dc\ud130 \ud50c\ub808\uc774\ud2b8\uc5d0 \ucd94\uac00\ud55c \ub2e4\uc74c \ud615\uad11\uacc4\ub97c \uc0ac\uc6a9\ud558\uc5ec \ud615\uad11\uc744 \uce21\uc815\ud569\ub2c8\ub2e4. \ubbfc\uac10\ud558\uace0 \uc815\ud655\ud55c QuantiFluor\u00ae dsDNA \uc2dc\uc2a4\ud15c\uc740 \ub2e8\uc77c \ud29c\ube0c\ubfd0\ub9cc \uc544\ub2c8\ub77c 96\uc6f0 \ubc0f 384\uc6f0 \ud50c\ub808\uc774\ud2b8\uc5d0\uc11c dsDNA\ub97c \uc815\ub7c9\ud654\ud558\ub294 \ub370 \uc0ac\uc6a9\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p><p>\uc18c\ub7c9\uc744 \ubc18\ubcf5\uc801\uc73c\ub85c \ud53c\ud3ab\ud305\ud558\ub294 \ub3d9\uc548 \uc815\ud655\uc131\uc744 \ubcf4\uc7a5\ud558\ub294 \uac83\uc740 \uc5b4\ub824\uc6b8 \uc218 \uc788\uc73c\uba70 \uc774 \uacfc\uc815\uc740 \ub178\ub3d9 \uc9d1\uc57d\uc801\uc774\uba70 \uc624\ub958\uc640 \ube44\uc6a9\uc774 \ub9ce\uc774 \ub4dc\ub294 \uc7ac\uc791\uc5c5\uc774 \uc218\ubc18\ub429\ub2c8\ub2e4. \ub530\ub77c\uc11c \ub354 \ub192\uc740 \ucc98\ub9ac\ub7c9\uc744 \ud544\uc694\ub85c \ud558\ub294 \ub300\ubd80\ubd84\uc758 \uc2e4\ud5d8\uc2e4\uc5d0\uc11c\ub294 \uc815\ub7c9 \ubd84\uc11d\uc744 \uc704\ud574 \uc790\ub3d9\ud654\ub41c \ub85c\ubd07 \uc561\uccb4 \ucc98\ub9ac \ud50c\ub7ab\ud3fc\uc744 \uc120\ud0dd\ud558\uace0 \uc788\uc2b5\ub2c8\ub2e4.MANTIS\u00ae\ub294 \ub300\ud615 \uc2dc\uc2a4\ud15c\ubcf4\ub2e4 \ud6e8\uc52c \uc801\uc740 \ube44\uc6a9\uc73c\ub85c \ub192\uc740 \ucc98\ub9ac\ub7c9\uc758 \uc2dc\uc57d \ub514\uc2a4\ud39c\uc2f1\uc5d0 \ud544\uc694\ud55c \ub9ce\uc740 \uc694\uad6c \uc0ac\ud56d\uc744 \ucda9\uc871\ud558\ub294 \uc0c8\ub85c\uc6b4 \ubca4\uce58\ud0d1 \uc561\uccb4 \ucc98\ub9ac\uae30\uc785\ub2c8\ub2e4. \ud575\uc0b0 \uc815\ub7c9\ud654\uc758 \uacbd\uc6b0, MANTIS\u00ae\ub294 \uc7ac\ud604 \uac00\ub2a5\ud55c \uc815\ub7c9\uc801 \uacb0\uacfc\ub97c \uc704\ud574 \uc18c\ub7c9\uc758 QuantiFluor\u00ae \uc5fc\ub8cc \uc791\uc5c5 \uc6a9\uc561\uc744 \uc815\ud655\ud558\uac8c \ub514\uc2a4\ud39c\uc2f1\ud569\ub2c8\ub2e4. \uc774 \uc560\ud50c\ub9ac\ucf00\uc774\uc158 \ub178\ud2b8\uc5d0\uc11c\ub294 96\uc6f0 \ubc0f 384\uc6f0 \ud50c\ub808\uc774\ud2b8 \ud615\uc2dd\uc758 QuantiFluor\u00ae dsDNA \uc2dc\uc2a4\ud15c\uc5d0 \ub300\ud55c \uc2dc\uc57d \ucc98\ub9ac\ub97c \ube60\ub974\uace0 \uc815\ud655\ud558\uac8c \uc790\ub3d9\ud654\ud558\ub294 MANTIS\u00ae \uc561\uccb4 \ud504\ub85c\uc138\uc11c\uc758 \ud638\ud658\uc131\uc744 \ubcf4\uc5ec\uc90d\ub2c8\ub2e4.<\/p><p>\u00a0<\/p><p><strong>\ud544\uc218 \uc790\ub8cc<\/strong><\/p><p style=\"padding-left: 40px;\">- MANTIS\u00ae \uae30\uae30(\ud3ec\ubbac\ub77c\ud2b8\ub9ad\uc2a4)<\/p><p style=\"padding-left: 40px;\">- \uace0\uc6a9\ub7c9 \uce69(\ud3ec\ubbac\ub77c\ud2b8\ub9ad\uc2a4 Cat.# MCHS6)<\/p><p style=\"padding-left: 40px;\">- QuantiFluor\u00ae dsDNA \uc2dc\uc2a4\ud15c(Cat.# E2670)<\/p><p style=\"padding-left: 40px;\">- \ub274\ud074\ub808\uc544\uc81c\uac00 \uc5c6\ub294 \ubb3c(Cat.# P1195)<\/p><p style=\"padding-left: 40px;\">- \uac80\uc740\uc0c9 \ud3c9\ud3c9\ud55c \ubc14\ub2e5\uc758 96\uc6f0 \ud50c\ub808\uc774\ud2b8(\ucf54\ub2dd Cat.# 3915) \ub610\ub294 384\uc6f0 \ud50c\ub808\uc774\ud2b8(\ucf54\ub2dd Cat.# 3573)<\/p><p style=\"padding-left: 40px;\">- 15ml \ub610\ub294 50ml \uc6d0\ubfd4\ud615 \ud29c\ube0c<\/p><p style=\"padding-left: 40px;\">- \ud615\uad11 \uce21\uc815\uc744 \uc704\ud55c \uba40\ud2f0\uc6f0 \uac80\ucd9c\uae30(\uc608: GloMax Discover \uc2dc\uc2a4\ud15c [Cat.# GM3000])<\/p><p>\u00a0 \u00a0<\/p><p><strong>\uc2e4\ud5d8 \ud504\ub85c\uadf8\ub7a8<\/strong><\/p><p>\uc2dc\uc57d \uc900\ube44.\u00a0<\/p><ol><li><p>1X TE \ubc84\ud37c \uc900\ube44: 20X TE \ubc84\ud37c\ub97c \ub274\ud074\ub808\uc544\uc81c\uac00 \uc5c6\ub294 \ubb3c\ub85c 20\ubc30 \ud76c\uc11d\ud569\ub2c8\ub2e4.<\/p><\/li><li><p>QuantiFluor\u00ae dsDNA \uc5fc\ub8cc\uc758 \uc791\ub3d9 \uc6a9\uc561\uc744 \uc900\ube44\ud569\ub2c8\ub2e4.<\/p><p>a. 96\uc6f0 \ud615\uc2dd: 1X TE \ubc84\ud37c\uc5d0 QuantiFluor\u00ae dsDNA \uc5fc\ub8cc\ub97c 1:400\uc73c\ub85c \ud76c\uc11d\ud569\ub2c8\ub2e4. \uc790\uc138\ud55c \uc0ac\uc6a9 \uc9c0\uce68\uc740 QuantiFluor\u00ae dsDNA \uc2dc\uc2a4\ud15c \uae30\uc220 \uc124\uba85\uc11c #TM346\uc744 \ucc38\uc870\ud558\uc2ed\uc2dc\uc624.<\/p><p>b. 384\uc6f0 \ud615\uc2dd: \uc5fc\ub8cc \ud76c\uc11d\uc740 \uc6d0\ud558\ub294 \ubd84\uc11d \uc694\uad6c\uc0ac\ud56d\uc5d0 \ub530\ub77c \uacb0\uc815\ub429\ub2c8\ub2e4. (\uc801\uc808\ud55c \uc5fc\ub8cc \ud76c\uc11d\uc744 \uc120\ud0dd\ud558\ub294 \ubc29\ubc95\uc5d0 \ub300\ud55c \uc790\uc138\ud55c \ub0b4\uc6a9\uc740 QuantiFluor\u00ae dsDNA \uc2dc\uc2a4\ud15c\uc744 \uc0ac\uc6a9\ud55c \uace0\ucc98\ub9ac\ub7c9, \uc18c\ub7c9 DNA \uc815\ub7c9\ud654\ub97c \uc704\ud55c \uc560\ud50c\ub9ac\ucf00\uc774\uc158 \ub178\ud2b8\ub97c \ucc38\uc870\ud558\uc2ed\uc2dc\uc624. ) \uc5ec\uae30\uc11c\ub294 1X TE \ubc84\ud37c\uc5d0 1:200\uc73c\ub85c \ud76c\uc11d\ud55c QuantiFluor\u00ae dsDNA \uc5fc\ub8cc\ub97c \uc0ac\uc6a9\ud558\uc5ec 0.05-50ng DNA\ub97c \uc815\ub7c9\ud654\ud569\ub2c8\ub2e4.<\/p><\/li><li><p>\ud45c\uc900 \uace1\uc120 \uc900\ube44: \uc54c\ub824\uc9c0\uc9c0 \uc54a\uc740 \uc0d8\ud50c\uc758 \ub18d\ub3c4 \ubc94\uc704\ub97c \uc704\uc544\ub798\ub85c \ud655\uc7a5\ud558\ub294 \uc54c\ub824\uc9c4 \ub18d\ub3c4\uc758 dsDNA \ud45c\uc900\uacfc 1X TE \ubc84\ud37c\ub97c \uc0ac\uc6a9\ud558\uc5ec \uc5f0\uc18d \ud76c\uc11d\ud588\uc2b5\ub2c8\ub2e4. \ube14\ub7ad\ud06c \uc0d8\ud50c\uc758 \uacbd\uc6b0 1X TE \ubc84\ud37c\ub97c \uc0ac\uc6a9\ud569\ub2c8\ub2e4.<\/p><\/li><\/ol><p>\uae30\uae30 \uc124\uc815.<\/p><ol><li><p>MANTIS\u00ae \ub370\uc2a4\ud06c\ud1b1 \uc560\ud50c\ub9ac\ucf00\uc774\uc158\uc744 \uc5f4\uace0 \ud30c\uc77c &gt; \uc0c8 \ubc30\ud3ec \ubaa9\ub85d\uc744 \uc120\ud0dd\ud569\ub2c8\ub2e4. \ubaa9\ub85d\uc5d0\uc11c \uce21\uc815 \ubcf4\ub4dc\ub97c \uc120\ud0dd\ud569\ub2c8\ub2e4.<br \/><img fetchpriority=\"high\" decoding=\"async\" class=\"size-large wp-image-26050 alignnone\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/4-1024x551.png\" alt=\"\" width=\"1024\" height=\"551\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/4-1024x551.png 1024w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/4-300x161.png 300w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/4-768x413.png 768w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/4.png 1242w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/p><\/li><li><p>\uc18c\ud504\ud2b8\uc6e8\uc5b4\uac00 \uc0ac\uc6a9\uc790\uc5d0\uac8c \ub514\uc2a4\ud39c\uc2f1 \ubaa9\ub85d\uc5d0 \uc2dc\uc57d\uc744 \ucd94\uac00\ud558\ub77c\ub294 \uba54\uc2dc\uc9c0\ub97c \ud45c\uc2dc\ud569\ub2c8\ub2e4. \"\uc2dc\uc57d \uc774\ub984\" \ud544\ub4dc\uc5d0 \"QuantiFluor dsDNA\"\ub97c \uc785\ub825\ud558\uace0 \uc644\ub8cc\ub418\uba74 \"\ud655\uc778\"\uc744 \uc120\ud0dd\ud569\ub2c8\ub2e4.<br \/><img decoding=\"async\" class=\"size-large wp-image-26051 alignnone\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/5-1024x549.png\" alt=\"\" width=\"1024\" height=\"549\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/5-1024x549.png 1024w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/5-300x161.png 300w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/5-768x412.png 768w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/5.png 1242w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/p><\/li><li><p>\ub9c8\uc774\ud06c\ub85c\uc5b4\ub808\uc774 \uc704\uce58 #1\uc5d0 QuantiFluor dsDNA\ub97c \ud560\ub2f9\ud569\ub2c8\ub2e4.<br \/><img decoding=\"async\" class=\"size-large wp-image-26052 alignnone\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/6-1024x549.png\" alt=\"\" width=\"1024\" height=\"549\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/6-1024x549.png 1024w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/6-300x161.png 300w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/6-768x412.png 768w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/6.png 1242w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/p><\/li><li><p>\ub9c8\uc774\ud06c\ub85c\uc5b4\ub808\uc774 \uc704\uce58 #1\uc758 \uc2dc\uc57d \ub799\uc5d0 QuantiFluor\u00ae dsDNA \uc6cc\ud0b9 \uc6a9\uc561\uc744 \ub193\uc2b5\ub2c8\ub2e4. \uce69\uc5d0\uc11c \uc2dc\uc57d\uc73c\ub85c \ud29c\ube0c\ub97c \ub123\uc2b5\ub2c8\ub2e4.<\/p><\/li><li><p>\uc6d0\ud558\ub294 \uad6c\uba4d\uc744 \uc120\ud0dd\ud558\uace0 \uc120\ud0dd\ud55c \uad6c\uba4d\uc758 \ubaa9\ud45c \uc6a9\ub7c9\uc744 \uc785\ub825\ud558\uc5ec \uce69\uc744 \ucc44\uc6b0\uace0 \uc0c8\ub85c\uc6b4 \uc561\uccb4 \ub514\uc2a4\ud39c\uc2f1\uc744 \uc124\uc815\ud569\ub2c8\ub2e4.<\/p><p>a. 96\uc6f0 \ud615\uc2dd: \uac01 \uc6f0\uc5d0 200\u00b5l\uc758 \uc791\uc5c5 \uc6a9\uc561\uc744 \ubd84\uc8fc\ud569\ub2c8\ub2e4.<\/p><p>b. 384-\uc6f0 \ud615\uc2dd: \uac01 \uc6f0\uc5d0 36\u00b5l\uc758 \uc791\uc5c5 \uc6a9\uc561\uc744 \ubd84\uc8fc\ud569\ub2c8\ub2e4.<br \/><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-26053 alignnone\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/7-1024x548.png\" alt=\"\" width=\"1024\" height=\"548\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/7-1024x548.png 1024w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/7-300x161.png 300w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/7-768x411.png 768w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/7.png 1244w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/p><\/li><li><p>\ub9c8\uc774\ud06c\ub85c\ud50c\ub808\uc774\ud2b8\ub97c MANTIS\u00ae\ub370\ud06c\uc5d0 \ucd94\uac00\ud558\uace0 \uc2dc\uc791\uc744 \ub204\ub985\ub2c8\ub2e4.<\/p><\/li><\/ol><p>\u00a0<\/p><p>\ubd84\uc11d\uc801 \ud310\ub2e8:<\/p><ol><li><p>\ud45c\uc900 \uc2dc\ub8cc, \ube14\ub7ad\ud06c \ubc0f \uc54c \uc218 \uc5c6\ub294 \uc2dc\ub8cc\ub97c \uba40\ud2f0\uc6f0 \ud50c\ub808\uc774\ud2b8\uc758 \uac01 \uc6f0\uc5d0 \uc218\ub3d9\uc73c\ub85c \ud53c\ud3ab\ud305\ud569\ub2c8\ub2e4.<\/p><p>c. 96\uc6f0 \ud615\uc2dd: 1-20\u00b5l\uc758 \ud45c\uc900, \ube14\ub7ad\ud06c \ubc0f \uc54c \uc218 \uc5c6\ub294 \uc0d8\ud50c\uc744 \ucd94\uac00\ud569\ub2c8\ub2e4.<\/p><p>d. 384\uc6f0 \ud615\uc2dd: \ud45c\uc900, \ube14\ub7ad\ud06c \ubc0f \uc54c \uc218 \uc5c6\uc74c 4\u00b5l\ub97c \ucd94\uac00\ud569\ub2c8\ub2e4.<\/p><\/li><li><p>\ud50c\ub808\uc774\ud2b8 \uc170\uc774\ucee4\ub97c \uc0ac\uc6a9\ud558\uac70\ub098 \ud53c\ud3ab\ud305\uc744 \ud1b5\ud574 \uac01 \uc6f0\uc758 \uc561\uccb4\ub97c \ud53c\ud3ab\ud305\ud558\uc5ec \ub9c8\uc774\ud06c\ub85c\ud50c\ub808\uc774\ud2b8\ub97c \uc644\uc804\ud788 \ud63c\ud569\ud569\ub2c8\ub2e4. \uc644\uc804\ud788 \ud63c\ud569\ud558\uc9c0 \uc54a\uc73c\uba74 \uc6f0\ub9c8\ub2e4 \ud310\ub3c5\uac12\uc774 \ub2ec\ub77c\uc9c8 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \ub610\ud55c \ud615\uad11\uc744 \ubc29\ud574\ud560 \uc218 \uc788\ub294 \uae30\ud3ec\uac00 \uc720\uc785\ub418\uc9c0 \uc54a\ub3c4\ub85d \ud558\uc138\uc694.<\/p><\/li><li><p>\uc2e4\uc628\uc5d0\uc11c 5\ubd84\uac04 \ubd84\uc11d \uc2dc\uc57d\uc744 \ubc30\uc591\ud569\ub2c8\ub2e4.<\/p><\/li><li><p>\ud615\uad11(504nmEx\/531nmEm)\uc744 \uac10\uc9c0\ud569\ub2c8\ub2e4. GloMax\u00ae \uc2dc\uc2a4\ud15c \uac10\uc9c0 \uae30\uae30\ub97c \uc0ac\uc6a9\ud558\ub294 \uacbd\uc6b0, \ubbf8\ub9ac \ub85c\ub4dc\ub41c \ud504\ub85c\ud1a0\ucf5c\uc778 \"QuantiFluor dsDNA \uc2dc\uc2a4\ud15c\"\uc744 \uc120\ud0dd\ud569\ub2c8\ub2e4.<\/p><\/li><li><p>\uac01 \ud45c\uc900 \ubc0f \uc0d8\ud50c \ud615\uad11\uc5d0\uc11c \ube14\ub7ad\ud06c \uc0d8\ud50c(1X TE \ubc84\ud37c)\uc758 \ud615\uad11\uc744 \uc81c\uac70\ud558\uc5ec \ub2e4\uc74c\uacfc \uac19\uc774 dsDNA \ub18d\ub3c4\ub97c \uacc4\uc0b0\ud569\ub2c8\ub2e4. DNA \ud45c\uc900\uc758 \ubcf4\uc815 \ub370\uc774\ud130\ub97c \uc0ac\uc6a9\ud558\uc5ec \ud615\uad11 \ub300 DNA \ub18d\ub3c4\uc758 \ud45c\uc900 \uace1\uc120\uc744 \uc0dd\uc131\ud569\ub2c8\ub2e4. \uc120\ud615 \ub610\ub294 \ud30c\uc6cc \ud68c\uadc0\ub97c \ud1b5\ud574 \ud45c\uc900 \uace1\uc120\uc5d0\uc11c \uc54c \uc218 \uc5c6\ub294 \uc0d8\ud50c\uc758 DNA \ub18d\ub3c4\ub97c \uacb0\uc815\ud569\ub2c8\ub2e4.<\/p><\/li><\/ol><p>\ud45c\uc900\ud654.<\/p><ol><li>GloMax\u00ae \uac80\ucd9c \uae30\uae30\uc758 \ub18d\ub3c4 \uac12\uc744 MANTIS\u00ae \uc18c\ud504\ud2b8\uc6e8\uc5b4\ub85c \uc9c1\uc811 \uac00\uc838\uc640 \ud45c\uc900\ud654\uc5d0 \ud544\uc694\ud55c \ubd80\ud53c\ub97c \uacc4\uc0b0\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4(\uadf8\ub9bc 1 \ubc0f 2 \ucc38\uc870).<\/li><\/ol><p><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-26054 alignnone\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/8-1024x306.png\" alt=\"\" width=\"1024\" height=\"306\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/8-1024x306.png 1024w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/8-300x90.png 300w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/8-768x229.png 768w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/8.png 1038w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/p><p>\uadf8\ub9bc 1: 96\uc6f0 \ud50c\ub808\uc774\ud2b8(\uadf8\ub9bc A) \ubc0f 384\uc6f0 \ud50c\ub808\uc774\ud2b8(\uadf8\ub9bc B)\uc5d0\uc11c MANTIS\u00ae \uc561\uccb4 \ud504\ub85c\uc138\uc11c\ub97c \uc0ac\uc6a9\ud558\uc5ec QuantiFluor\u00ae dsDNA \uc5fc\ub8cc \uc791\uc5c5 \uc6a9\uc561\uc744 \ub514\uc2a4\ud39c\uc2f1\ud558\ub294 K562 \uc778\uac04 \uac8c\ub188 DNA \ud45c\uc900(Cat.# E4931)\uc758 \uc815\ub7c9\ud654. \ud615\uad11\uc740 \uae00\ub85c\ub9e5\uc2a4 \ub514\uc2a4\ucee4\ubc84 \uc2dc\uc2a4\ud15c\uc5d0\uc11c \uce21\uc815\ub418\uc5c8\uace0 \uc54c \uc218 \uc5c6\ub294 \uc0d8\ud50c\uc740 \ud30c\uc6cc \ud68c\uadc0 \ud45c\uc900 \uace1\uc120 \ub0b4\uc5d0\uc11c \uc0bd\uc785\ub418\uc5c8\uc2b5\ub2c8\ub2e4.<\/p><p><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-26055 alignnone\" src=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/9.png\" alt=\"\" width=\"1016\" height=\"320\" srcset=\"https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/9.png 1016w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/9-300x94.png 300w, https:\/\/www.accscicn.com\/wp-content\/uploads\/2022\/02\/9-768x242.png 768w\" sizes=\"(max-width: 1016px) 100vw, 1016px\" \/><\/p><p>\uadf8\ub9bc 2: mRNA \uc804\uc0ac\uccb4\uc758 \ud3c9\uade0 \ud06c\uae30\ub97c \uc2dc\ubbac\ub808\uc774\uc158\ud558\ub294 \ub370 \uc0ac\uc6a9\ub41c 2,200bp PCR \uc0b0\ubb3c\uc758 \uc815\ub7c9\ud654. 96\uc6f0 \ud50c\ub808\uc774\ud2b8 \ud615\uc2dd(\uadf8\ub9bc A) \ubc0f 384\uc6f0 \ud50c\ub808\uc774\ud2b8 \ud615\uc2dd(\uadf8\ub9bc B)\uc73c\ub85c QuantiFluor\u00ae dsDNA \uc2dc\uc2a4\ud15c\uc744 \ub514\uc2a4\ud39c\uc2f1\ud558\uae30 \uc704\ud574 Mantis \uc561\uccb4 \ud504\ub85c\uc138\uc11c\ub97c \uc0ac\uc6a9\ud588\uc2b5\ub2c8\ub2e4. \ud615\uad11\uc740 \uae00\ub85c\ub9e5\uc2a4 \ub514\uc2a4\ucee4\ubc84 \uc2dc\uc2a4\ud15c\uc5d0\uc11c \uce21\uc815\ub418\uc5c8\uace0, \uc54c \uc218 \uc5c6\ub294 \uc0d8\ud50c\uc740 \ud30c\uc6cc \ud68c\uadc0 \ud45c\uc900 \uace1\uc120 \ub0b4\uc5d0\uc11c \uc0bd\uc785\ub418\uc5c8\uc2b5\ub2c8\ub2e4.<\/p><p>\u00a0<\/p><p><strong>\uc694\uc57d<\/strong><\/p><p>MANTIS\u00ae \uc561\uccb4 \ud504\ub85c\uc138\uc11c\ub294 \uc5f0\uad6c\uc790\uc5d0\uac8c \ub2e4\uc591\ud55c \uc2e4\ud5d8 \ud504\ub85c\ud1a0\ucf5c\uc744 \uba87 \ubd84 \uc548\uc5d0 \uad6c\ucd95\ud560 \uc218 \uc788\ub294 \uc720\uc5f0\ud55c \uc6cc\ud06c\uc2a4\ud14c\uc774\uc158\uc744 \uc81c\uacf5\ud569\ub2c8\ub2e4. \uc774\ub7ec\ud55c \ud504\ub85c\ud1a0\ucf5c\uc744 \uc815\ud655\ud558\uac8c \uc2e4\ud589\ud558\uc5ec \uc2e4\ud5d8\uc2e4 \ud6a8\uc728\uc131\uacfc \uc2e4\ud5d8 \uc7ac\ud604\uc131\uc744 \uac1c\uc120\ud558\uc138\uc694. \uc774 \uc560\ud50c\ub9ac\ucf00\uc774\uc158 \ub178\ud2b8\ub294 \uc790\ub3d9\ud654 \ud50c\ub7ab\ud3fc\uc73c\ub85c \ube60\ub974\uace0 \uc6d0\ud65c\ud558\uac8c \uc804\ud658\ud560 \uc218 \uc788\ub294 \uc561\uccb4 \ucc98\ub9ac \uc18c\ud504\ud2b8\uc6e8\uc5b4\uc758 \uc0ac\uc6a9 \ud3b8\uc758\uc131\uc744 \ubcf4\uc5ec\uc90d\ub2c8\ub2e4. MANTIS\u00ae \uc561\uccb4 \ucc98\ub9ac\uae30\ub97c \uc0ac\uc6a9\ud558\uc5ec QuantiFluor\u00ae dsDNA \uc2dc\uc2a4\ud15c\uc5d0 \uc2dc\uc57d\uc744 \ucd94\uac00\ud558\uba74 \uae30\uae30 \ud504\ub85c\uadf8\ub798\ubc0d\uc774 \uac70\uc758 \ub610\ub294 \uc804\ud600 \uc5c6\uc774 \ub2e4\uce35 \ub9c8\uc774\ud06c\ub85c\uc2dc\ud130 \ud50c\ub808\uc774\ud2b8\uc5d0\uc11c \uc7ac\ud604 \uac00\ub2a5\ud55c \uc815\ub7c9\ud654\uac00 \uac00\ub2a5\ud569\ub2c8\ub2e4.<\/p><p>\u00a0<\/p><p>\ucd9c\ucc98: @BostonFummerle<\/p><p>\uc2dc\uac04: 2022.02.15<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-1bbf8b72\" data-id=\"1bbf8b72\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4fe93b6c elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"4fe93b6c\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.21.0 - 20-05-2024 *\/\n.elementor-widget-divider{--divider-border-style:none;--divider-border-width:1px;--divider-color:#0c0d0e;--divider-icon-size:20px;--divider-element-spacing:10px;--divider-pattern-height:24px;--divider-pattern-size:20px;--divider-pattern-url:none;--divider-pattern-repeat:repeat-x}.elementor-widget-divider .elementor-divider{display:flex}.elementor-widget-divider .elementor-divider__text{font-size:15px;line-height:1;max-width:95%}.elementor-widget-divider .elementor-divider__element{margin:0 var(--divider-element-spacing);flex-shrink:0}.elementor-widget-divider .elementor-icon{font-size:var(--divider-icon-size)}.elementor-widget-divider .elementor-divider-separator{display:flex;margin:0;direction:ltr}.elementor-widget-divider--view-line_icon .elementor-divider-separator,.elementor-widget-divider--view-line_text .elementor-divider-separator{align-items:center}.elementor-widget-divider--view-line_icon .elementor-divider-separator:after,.elementor-widget-divider--view-line_icon .elementor-divider-separator:before,.elementor-widget-divider--view-line_text .elementor-divider-separator:after,.elementor-widget-divider--view-line_text .elementor-divider-separator:before{display:block;content:\"\";border-block-end:0;flex-grow:1;border-block-start:var(--divider-border-width) var(--divider-border-style) var(--divider-color)}.elementor-widget-divider--element-align-left .elementor-divider .elementor-divider-separator>.elementor-divider__svg:first-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-left .elementor-divider-separator:before{content:none}.elementor-widget-divider--element-align-left .elementor-divider__element{margin-left:0}.elementor-widget-divider--element-align-right .elementor-divider .elementor-divider-separator>.elementor-divider__svg:last-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-right .elementor-divider-separator:after{content:none}.elementor-widget-divider--element-align-right .elementor-divider__element{margin-right:0}.elementor-widget-divider--element-align-start .elementor-divider .elementor-divider-separator>.elementor-divider__svg:first-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-start .elementor-divider-separator:before{content:none}.elementor-widget-divider--element-align-start .elementor-divider__element{margin-inline-start:0}.elementor-widget-divider--element-align-end .elementor-divider .elementor-divider-separator>.elementor-divider__svg:last-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-end .elementor-divider-separator:after{content:none}.elementor-widget-divider--element-align-end .elementor-divider__element{margin-inline-end:0}.elementor-widget-divider:not(.elementor-widget-divider--view-line_text):not(.elementor-widget-divider--view-line_icon) .elementor-divider-separator{border-block-start:var(--divider-border-width) var(--divider-border-style) var(--divider-color)}.elementor-widget-divider--separator-type-pattern{--divider-border-style:none}.elementor-widget-divider--separator-type-pattern.elementor-widget-divider--view-line .elementor-divider-separator,.elementor-widget-divider--separator-type-pattern:not(.elementor-widget-divider--view-line) .elementor-divider-separator:after,.elementor-widget-divider--separator-type-pattern:not(.elementor-widget-divider--view-line) .elementor-divider-separator:before,.elementor-widget-divider--separator-type-pattern:not([class*=elementor-widget-divider--view]) .elementor-divider-separator{width:100%;min-height:var(--divider-pattern-height);-webkit-mask-size:var(--divider-pattern-size) 100%;mask-size:var(--divider-pattern-size) 100%;-webkit-mask-repeat:var(--divider-pattern-repeat);mask-repeat:var(--divider-pattern-repeat);background-color:var(--divider-color);-webkit-mask-image:var(--divider-pattern-url);mask-image:var(--divider-pattern-url)}.elementor-widget-divider--no-spacing{--divider-pattern-size:auto}.elementor-widget-divider--bg-round{--divider-pattern-repeat:round}.rtl .elementor-widget-divider .elementor-divider__text{direction:rtl}.e-con-inner>.elementor-widget-divider,.e-con>.elementor-widget-divider{width:var(--container-widget-width,100%);--flex-grow:var(--container-widget-flex-grow)}<\/style>\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c74ffba elementor-widget elementor-widget-heading\" data-id=\"c74ffba\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\uad00\ub828 \uc81c\ud488<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-41ad45e9 elementor-grid-1 elementor-grid-tablet-2 elementor-grid-mobile-1 elementor-posts--thumbnail-top elementor-widget elementor-widget-posts\" data-id=\"41ad45e9\" data-element_type=\"widget\" data-settings=\"{&quot;classic_columns&quot;:&quot;1&quot;,&quot;classic_columns_tablet&quot;:&quot;2&quot;,&quot;classic_columns_mobile&quot;:&quot;1&quot;,&quot;classic_row_gap&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:35,&quot;sizes&quot;:[]},&quot;classic_row_gap_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;classic_row_gap_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" 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